Journal: Horticulture Research
Article Title: PpERF3 positively regulates ABA biosynthesis by activating PpNCED2/3 transcription during fruit ripening in peach
doi: 10.1038/s41438-018-0094-2
Figure Lengend Snippet: a Agrobacterium tumefaciens having PpNCED2/3 or PpERF3 plasmids was infiltrated into tobacco leaves to analyze the activity enhancement of PpNCED2/3 promoters by PpERF3. Significantly higher LUC/REN ratios were obtained with the PpERF3 effect vector than with the non-PpERF3 control vector, indicating that PpERF3 enhanced PpNCED2/3 promoter activity. b The PpERF3 effector with plasmid having the PpNCED2/3 promoters was infiltrated into tobacco leaves. The effector and empty pK2GW7 plasmids that were co-transformed into tobacco were used as controls. Values are means ±SD (n=3), * and ** represent significance at p<0.05 and p<0.01, compared to control based on t-test, respectively
Article Snippet: A dual-luciferase assay kit was used to measure LUC and REN luciferase activity, and analysis was performed using the SpectraMax ® i3x Platform (USA) at 560 and 465 nm, respectively.
Techniques: Activity Assay, Plasmid Preparation, Transformation Assay